Reference Space Estimation 
CAVALIERI PRINCIPLE
Bonaventura Francesco Cavalieri 
 (1598 – 1647)
 He was an Italian mathematician.
 He is known for his work on the problems of optics and motion, work on the precursors of infinitesimal calculus, and the introduction of logarithms toItaly.
Cavalieri's principle in geometry partially anticipated integral calculus.
REFERENCE SPACE ESTIMATION
CAVALIERI METHOD:
V(structure)=T.A
Volume of the structure is estimated by summing the areas (A)  and multiplying by the distance between sampled sections (T).
 
IMMERSION METHOD:
Volume = W2-W1 / special weight
Special weight of normal saline =1.0048
V = T . a/p . ΣP
a/p=ΔX. ΔY/ Mag2
Magnification=
Final image length / Micrometer length
 
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Shiraz University Medical Sciences
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