Numerical Density  
Number Estimation    
       Disector Principle (Reference and
       Look-up Sections, Unbiased Counting Frame)
Physical Disector:
Rule of Thumb: The distance between the disector sections should be about 30% of the average height of the object to be counted.
     Optical Disector
Nv= ΣQ / V      
Nv = ΣQ / h. (a/f). ΣP.(t/BA)
   2-5 counts in each disector
Optical Fractionator:
 N = 1/ssf×1/asf×1/tsf×∑ Q
 
 Histomorphometry & Stereology Research Center
Shiraz University Medical Sciences
Postal code: 71348 - 45794
Tel & Fax: + 98 71 32304372    

 
 
 
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